Bio panning of human stem cell factor(2) mimetic peptides from phage displayed random peptide library.
- Author:
Lin SU
1
;
Yan KONG
;
Chang-zheng LIU
;
Ke-gong YANG
;
Song-sen CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Humans; Peptide Library; Peptides; genetics; isolation & purification; Stem Cell Factor; genetics; isolation & purification
- From: Acta Academiae Medicinae Sinicae 2011;33(4):351-356
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo screen human stem cell factor (hSCF) mimetic peptides in vitro with a phage-display random peptide library.
METHODSPhage clones with high hSCF receptor (rc-kit/Ig 1-3)-binding activity was screened from phage-displayed random hepta/dodecapeptide library by phage enzyme-linked immunosorbent assay (ELISA). Phage single DNA was extracted and sequenced. Four kinds of peptide with higher c-Kit/Ig 1-3 binding activity were chosen for synthesis and characterized by using cell proliferation assay with 3-(4,5-dimethylthiazol-2-yl)2,5-diphenyltetrazolium bromide (MTT) method in UT-7 cells.
RESULTSEleven Ph.D.-C7C clones and eight Ph.D-12 phage clones with high hSCF receptor-binding activity were selected from phage-displayed random hepta/dodecapeptide library, respectively. Sequence analysis showed there were no homologous sequence between hSCF and these screened mimetic peptides except one homologous sequence DPSPHTH found in heptapeptide library. All these four synthesized peptides (CE3, CE16, LE4, and LE20), particularly CE16 and LE20, stimulated UT-7 cell proliferation.
CONCLUSIONFour hSCF mimetic peptides were successfully isolated from phage-displayed random peptide library..
