Biological features and ultrastructure of human umbilical cord mesenchymal stem cells.
- Author:
Shao-fang ZHU
1
;
Yuan-li HE
;
Xia-fei FU
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; Cell Cycle; Cell Proliferation; Cells, Cultured; Hepatocyte Growth Factor; metabolism; Humans; Insulin-Like Growth Factor I; metabolism; Mesenchymal Stromal Cells; cytology; metabolism; ultrastructure; Umbilical Cord; cytology; Vascular Endothelial Growth Factor A; metabolism
- From: Acta Academiae Medicinae Sinicae 2011;33(4):382-386
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo isolate and culture human umbilical cord mesenchymal stem cells (MSCs) and explore their biological features and ultrastructure.
METHODSAfter isolating MSCs from the human umbilical cord, the proliferation, cycle, and apoptosis were observed. The cell ultrastructure was observed under transmission electron microscope. The cytokines including vascular endothelial growth factor (VEGF), hepatocyte growth factor (HGF), and insulin-like growth factor-1 (IGF-1) were detected using enzyme-linked immunosorbent assay.
RESULTSHuman umbilical cord MSCs had fibroblast-like morphology and increased proliferation capability. Ultrastructural analysis showed that the MSCs had active cellular metabolism and strong migration and differentiation capabilities. Meanwhile, they could secrete anti-apoptotic cytokines such as VEGF, IGF-1, and HGF.
CONCLUSIONHuman umbilical cord MSCs can secrete many anti-apoptotic cytokine and have good biological features.
