Screening the hALR-interacting protein from the cDNA library of hepatocarcinoma cells and studying its biological functions.
- Author:
Jian-chuan DENG
1
;
Lin ZHANG
;
Hang SUN
;
Lin TANG
;
Na WANG
;
Qi LIU
Author Information
- Publication Type:Journal Article
- MeSH: Carcinoma, Hepatocellular; metabolism; pathology; Gene Library; Humans; Liver Neoplasms; metabolism; pathology; Liver Regeneration; Peptide Library; Peptides; metabolism; Protein Binding; Proteins; genetics; isolation & purification; metabolism; Tumor Cells, Cultured
- From: Chinese Journal of Hepatology 2006;14(5):346-349
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVESTo screen the hALR-interacting protein by phage-displayed technique and identify its biological activities.
METHODSThe specific phage clones that interacted with target protein hALR from a cDNA library of hepatocarcinoma cells were selected using the T7 phage-displayed technique. The acquired cDNA inserts were sequenced and analyzed by bioinformatic tools. The biological activities of the phage-displayed peptide affecting QGY hepatocarcinoma cells were studied using 3H-TdR method.
RESULTSThe cDNA inserts with 212 bp were acquired after 4 rounds of biopanning. They showed 100% homology with citron kinase. The phage-displayed peptide and the peptide combined with hALR affected QGY cells proliferation.
CONCLUSIONShALR-interacting peptide can be specifically screened by phage-displayed technique. Citron kinase that interacted with hALR potentially plays an important role in the proliferation of hepatocarcinoma cells.
