Transfection of pemt-2-cDNA inhibits the expression of cell cycle related proteins in rat CBRH-7919 hepatoma cells.
- Author:
Cui-ping LIU
1
;
Wei ZOU
;
Liang WANG
;
Zhao-chun CUI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; physiology; Caspase 3; biosynthesis; genetics; Cyclin-Dependent Kinase 2; biosynthesis; genetics; Cyclin-Dependent Kinase 4; biosynthesis; genetics; Cyclin-Dependent Kinases; biosynthesis; genetics; Down-Regulation; Liver Neoplasms, Experimental; genetics; metabolism; Phosphatidylethanolamine N-Methyltransferase; genetics; metabolism; Rats; Transfection; Tumor Cells, Cultured
- From: Chinese Journal of Hepatology 2006;14(5):350-352
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVESTo unravel the molecular mechanism of proliferation inhibition induced by transfection of pemt2-cDNA into rat CBRH-7919 hepatoma cells.
METHODSWe started with the highly expressed PEMT2 clone. Cell culture and Western blotting techniques were used to examine the expression of cyclinD1/CDK4, cyclinE/CDK2, phospho-Rb, caspase-3, c-jun and caveolins.
RESULTSOur results showed that CDK4, CDK2, phospho-Rb and c-jun were down regulated in the pemt2 highly expressed cell clone. The high expression clone of pemt2-transfected cells also showed over expression of caspase-3.
CONCLUSIONThe reductions of proliferation and apoptosis of pemt2 transfected cells could be related to the G1 phase arrest induced by down-regulation of the cell cycle-associated proteins.
