An analysis of the features of HBx protein distributed in liver cells and its expression in E. coli.
- Author:
Ju-qiang HAN
1
;
Li-hua DING
;
Bin YUAN
;
Xiao-hui WANG
;
Kang NING
;
Jie-zhi LI
;
Qiu-jun LU
;
Xiao YANG
;
Cui-fen HUANG
;
Qi-nong YE
Author Information
- Publication Type:Journal Article
- MeSH: Carcinoma, Hepatocellular; pathology; Cell Line; Cloning, Molecular; Escherichia coli; metabolism; Genetic Vectors; Glutathione Transferase; biosynthesis; genetics; Hepatocytes; cytology; metabolism; Humans; Liver; cytology; Liver Neoplasms; pathology; Mutation; Recombinant Fusion Proteins; biosynthesis; genetics; Trans-Activators; biosynthesis; genetics; Tumor Cells, Cultured
- From: Chinese Journal of Hepatology 2006;14(6):441-444
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the features of HBx protein distributed in liver cells and its expression in E. coli.
METHODSThe expression vectors encoding the full length HBx and its mutants were constructed by the routine molecular cloning method. HBx protein expression was detected using Western blotting. The distribution feature of HBx protein in liver cells was examined using the fluorescence confocal microscopy. A series of purified HBx fusion proteins were obtained by glutathione-sepharose 4B affinity chromatography.
RESULTSThe expression vectors were successfully constructed for the full length HBx and its mutants. HBx was found distributed uniformly in the nuclei but granularly in the cytoplasm of the liver cells. Under optimal conditions, the mutant GST-HBx (72-120aa) was easily degraded.
CONCLUSIONThis study may provide a basis for further study on the biological function of HBx at the protein level.
