Effects of c-met-siRNA on the growth and invasion of hepatocellular carcinoma MHCC97-H cells.
- Author:
Bin XIE
1
;
De-guo TANG
;
Jia-hong DONG
Author Information
- Publication Type:Journal Article
- MeSH: Carcinoma, Hepatocellular; genetics; metabolism; pathology; Cell Line, Tumor; Humans; Liver Neoplasms; pathology; Neoplasm Invasiveness; Neoplasm Metastasis; Plasmids; Proto-Oncogene Proteins c-met; genetics; metabolism; RNA, Small Interfering
- From: Chinese Journal of Hepatology 2006;14(7):499-504
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effects of c-met-siRNA on the growth and invasion of hepatocellular carcinoma MHCC97-H cells by pSuppressorRetro/c-met-siRNA recombinant plasmid transfection.
METHODSRecombinant plasmid transfection to Phoenix A cells was constructed using the lipofectin method and then the retrovirals containing c-met-siRNA were used to infect target cells MHCC97-H. In vitro, c-met expression was tested by Western blot. Cell proliferation, motility and invasiveness were studied using MTT, cell migration assay, and cell invasion assay, respectively.
RESULTSThe expression of c-met decreased significantly in MHCC97-H cells, and the most effective site of the target sequence was at 537. The growth, motility and invasiveness of MHCC97-H cells were inhibited.
CONCLUSIONThe results indicate that c-met-siRNA can down-regulate the expression of c-met and inhibit hepatocellular carcinoma cell proliferation, motility and invasiveness.
