The relationship between histone acetylation modification and IFN-gamma responsive gene regulation.
- Author:
Jin-jun GUO
1
;
Ying HUANG
;
Jun ZHANG
;
Ai-long HUANG
Author Information
- Publication Type:Journal Article
- MeSH: Acetylation; Chemokine CXCL10; metabolism; Gene Expression Regulation; HeLa Cells; Histone Deacetylases; genetics; metabolism; Histones; genetics; metabolism; Humans; Interferon-gamma; genetics; metabolism; RNA, Messenger; genetics
- From: Chinese Journal of Hepatology 2006;14(7):525-528
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVESTo study the role of histone modification in the regulation of IFN-gamma-activated gene using chromatin immunoprecipitation technique.
METHODSReal-time PCR was used to measure the mRNA level of interferon-gamma-inducible protein 10 (IP-10) in Hela cells. Chromatin immunoprecipitation combined with Real-time PCR was used to check the histone H4 acetylation level at IFN-stimulated response element (ISRE) locus of IP-10 gene.
RESULTSIP-10 was strongly activated by IFN-gamma. The histone H4 deacetylation happened at the ISRE locus when IP-10 was induced by IFN-gamma. The activation of IP-10 and the deacetylation of histone H4 at the ISRE site induced by IFN-gamma were inhibited or blocked by histone deacetylase (HDAC) inhibitor trichostatin A (TSA).
CONCLUSIONThe histone H4 deacetylation at the ISRE site is related with the activation of IP-10 by IFN-gamma.
