Coexpression of hepatitis B virus X gene and hepatitis C virus C gene in HepG2 cells and its effect on the expression of VEGF.
- Author:
Chong-yang LIU
1
;
Wei-wen LIU
;
Dong-feng CHEN
;
Jun WANG
Author Information
- Publication Type:Journal Article
- MeSH: Gene Expression; Gene Expression Regulation, Viral; Genetic Vectors; Hep G2 Cells; Hepacivirus; genetics; Humans; Trans-Activators; genetics; Transfection; Vascular Endothelial Growth Factor A; metabolism; Viral Core Proteins; genetics
- From: Chinese Journal of Hepatology 2006;14(7):529-531
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish an experimental model of HCV C-HBV X co-expression protein and explore its effect on the expression of VEGF.
METHODSThe HBV X gene was recovered by enzyme excision and inserted into PBK-CMV and PBK-HCVC, and recombinant plasmids PBK-X and PBK-X-C were constructed. The plasmids PBK-CMV, PBK-X, PBK-HCVC and PBK-X-C were transfected into HepG2 cells with liposomes. After being selected by G418, resistant colonies were obtained. Reverse transcription PCR and Western blot were used to show HBV X and HCV core protein expression. VEGF was analyzed using immunohistochemical methods and Western blot.
RESULTSThe recombinant plasmid PBK-X-C expressed HBV X and HCV core protein efficiently under the control of the vectors promoter. VEGF and VEGF mRNA of the cells co-expressing HCV C-HBV X proteins were higher than those cells expressing HBV X, HCV C and vector alone.
CONCLUSIONHBV X-HCV C co-expression protein can increase the expression of VEGF of HepG2 cells. It suggests that HBV and HCV have a synergic action in the carcinogenesis.
