Screening of proteins interacting with hepatitis C virus NS3 from T7-phage display library.
- Author:
Ying HUANG
1
;
Xue-fei CAI
;
Mao-rui HE
;
Jun ZHANG
;
Ai-long HUANG
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line; Gene Library; Hepacivirus; metabolism; Humans; Peptide Library; Protein Interaction Mapping; methods; Viral Fusion Proteins; genetics; isolation & purification; metabolism; Viral Nonstructural Proteins; genetics; metabolism
- From: Chinese Journal of Hepatology 2006;14(8):561-564
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo screen and identify proteins that interact with hepatitis C virus NS3 by means of T7-phage display system.
METHODSHepatitis C virus NS3 was expressed by prokaryotic expression and used as a selected molecule to biopan the T7 select human liver cDNA library; the selected positive clones were identified using DNA sequencing and analyzed with BLAST program in GenBank.
RESULTSAfter BLAST analysis in all the positive clones, the proteins which interacted with the hepatitis C virus NS3 were found to be serpin peptidase inhibitor, clade A, member 1 (SERPINA1) and cyclophilin-LC.
CONCLUSIONT7-phage display system is a convenient, rapid and effective method for screening interacting proteins. The proteins thus selected will provide an important means for studying the pathogenesis and carcinogenesis of HCV.
