Expression of anti-HBc single-chain variable fragment mediated by recombinant replication defective adenovirus in vitro.
- Author:
Zheng-hao TANG
1
;
Yong-sheng YU
;
Hui-hui MA
;
Hong JIANG
;
Min XI
;
Guo-qing ZANG
;
Ji-lu YAO
Author Information
- Publication Type:Journal Article
- MeSH: Adenoviridae; genetics; Cell Line; Hepatitis B Antibodies; genetics; immunology; Hepatitis B virus; genetics; immunology; Humans; Single-Chain Antibodies; genetics; immunology; Transfection
- From: Chinese Journal of Hepatology 2006;14(8):587-589
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVESTo define the expression of single-chain variable fragment (ScFv) against hepatitis B virus core protein (HBc) mediated by recombinant replication defective adenovirus carrying the anti-HBc ScFv gene in vitro and to define the activity of anti-HBc ScFv combining HBcAg.
METHODSThe recombinant adenoviruses carrying anti-HBc ScFv gene generated by homologous recombination in bacteria and packaged in 293 cells were transfected into HepG2 cells, and the anti-HBc ScFv was detected using SDS-PAGE and Western blot.
RESULTSGreen fluorescent protein (GFP) was observed in HepG2 cells after the transfection. SDS-PAGE displayed a protein strap about 2.7 x 10(4), and the result of Western blot displayed a positive reactive strap.
CONCLUSIONAnti-HBc ScFv can be expressed in cells mediated by recombinant replication defective adenovirus carrying the anti-HBc ScFv gene.
