Platelet-derived growth factor-BB inhibited p21~(WAF1) expression partially through transforming growth factor-β signalling system in vascular smooth muscle cell
10.3760/cma.j.issn.0253-3758.2010.02.016
- VernacularTitle:血小板衍生生长因子-BB通过转化生长因子-β信号系统调节大鼠血管平滑肌细胞内p21~(WAF1)表达
- Author:
Da-Bin PAN
1
;
Yong-Sheng KE
;
Wen-Jie LIU
;
You-Quan WEI
;
Jun TANG
;
Heng CAO
Author Information
1. 皖南医学院附属弋矶山医院
- Keywords:
Blood vessels;
Myocytes,smooth muscle;
Platelet-derived growth factor;
Transforming growth factor betal;
Cyclin-dependent kinase inhibitor p21
- From:
Chinese Journal of Cardiology
2010;38(2):160-165
- CountryChina
- Language:Chinese
-
Abstract:
Objective To assess if the modulating effect of platelet-derived growth factor(PDGF)BB on p21~(WAF1) was mediated by upregulating transforming growth factor(TGF)-β_1 expression in vascular smooth muscle cells(VSMC).Methods TGF-β_1 mRNA and protein expressions were measured by reverse transeription-PCR and ELISA,the protein expressions of p21~(WAF1) and the downstream TGF-βsignalling including TGF-β type I receptor(ALK-5 in VSMC),Smurf2,pSmad2/3,Smad4,Smad7 were detected by Western blot.Results PDGF-BB significantly upregulated the expressions of TGF-β_1 at mRNA(0.79-fold)and protein(1.98-fold)levels in VSMC,significantly inhibited the expression of p21WAF1(-67±12)%,and enhanced the expressions of ALK-5,pSmad2/3,Smad4,Smurf2 protein by 1.21-fold.0.95-fold,0.69.fold and 2.55-fold respectively,inhibited Smad7 expression(-65±9)%,these alterations were partially restored by anti-TGF-β_1 neutralizing antibody.Conclusions These findings suggested that PDGF-BB inhibited p21~(WAF1) expression in VSMC partially through upregulating TGF-β_1 expression via PDGFBB and TGF-β signalling pathways.
