Single Fe_2O_3-PLL labeled mouse spleen-derived endothelial progenitor cell detection by 7.0T MR system
10.3760/cma.j.issn.0253-3758.2010.02.017
- VernacularTitle:小鼠脾源性内皮祖细胞培养及7.0T磁共振单细胞成像初探
- Author:
Zhen-Yu JIA
1
;
Jun CHEN
;
Gao-Jun TENG
Author Information
1. 东南大学附属中大医院
- Keywords:
Stem cells;
Cell culture technique;
Magnetic resonance imaging
- From:
Chinese Journal of Cardiology
2010;38(2):166-170
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the feasibility of Single Fe_2O_3-PLL labeled mouse spleen-derived endothelial progenitor cells(EPCs)detection by 7.0T MR system.Methods Mononuclear cells(MNCs)were isolated from mouse spleen by density gradient centrifugation and EPCs were obtained by the difierent adherence of cells.Immunocytochemistry and fluorescent staining were performed to identify EPCs.The EPCs were labeled with Fe_2O_3-PLL and the intracellular iron was identified with prussian blue staining.MTr assay was assessed to evaluate proliferation of Fe_2O_3-PLL labeled EPCs.The cells underwent MR imaging with different sequences.Results Cultured in vitro,mouse spleen-derived MNCs resulted in EC-like morphology.These cells expressed EPCs-specie antigens,such as CD31,CD34 and vWF,and had the ability to incorporate ac-LDL and bind UEA-1.Between Fe_2O_3-PLL labeled EPCs and unlabeled cells,MTT value of light absorption had no statistical significant difference(day4 t=2.81,days t=-1.87,day6 t=-0.298,day7 t=-0.115,all P＞0.05).The signal void induced by labeled single cell is 20.2 pixels in MSME Sequence,and 20.2 pixeis in 3D-FLASH sequence(t=15.2,P＜0.05).Single cell could be detected by 7.0 T MR system.Conclusion MNCs isolated from mouse spleen can differentiate into endothelial cells in vitro and have the specific property of stem cells.The mouse spleen-derived EPCs can be labeled with Fe_2O_3-PLL efficiently.The labeled EPCs can be imaged as disoersed single cells.
