Effects of curcumin on sarcoplasmic reticulum Ca~(2+) -ATPase in rabbits with heart failure
10.3760/cma.j.issn.0253-3758.2010.04.022
- VernacularTitle:姜黄素对心力衰竭兔肌浆网钙泵表达的影响
- Author:
Yan ZHANG
1
;
Guo-Sheng LIN
;
Ming-Wei BAO
;
Xin-Ying WU
;
Che WANG
;
Bo YANG
Author Information
1. 武汉大学人民医院
- Keywords:
Heart failure,congestive;
Sarcoplasmic reticuhm calcium-transporting ATPases;
Cureumin
- From:
Chinese Journal of Cardiology
2010;38(4):369-373
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of curcumin on sarcoplasmic reticulum Ca~(2+)-ATPase in heart failure rabbits.Methods Rabbit heart failure model was made with aortic regurgitation and abdominal aorta constriction and 40 rabbits were randomly divided into 4 groups including:(1) heart failure treated with curcumin;(2) heart failure treated with placebo;(3) healthy control treated with curcumin and (4) healthy control treated with placebo.All rabbits were administrated with curcumin capsules or placebo capsules 100 mg·kg~(-1)·d~(-1),respectively.All groups were sacrificed after eight weeks.Myocardial ultrastructural organization was detected by transmission electron microscope.RT-PCR and Western blot were used to measure the expression of sarcoplasmic reticulum Ca~(2+)-ATPase in mRNA and protein levels,respectively.Malachite green colorimetric assay was used to evaluate the activity of sarcoplasmic reticulum Ca~(2+) -ATPase.Results All detected parameters were similar between control curcumin group and control placebo group.Compared with the control groups (Groups 3 and 4),the heart/body weight ratio was significantly increased in the heart failure-curcumin group (Group 1) and the heart failure-placebo group (Group 2,all P＜0.05),but the ratio was significantly lower in heart failure-curcumin group than in heart failure-placebo group (P＜0.05).The degree of heart failure was decreased by curcumin.Activity and mRNA and protein expression for sarcoplasmic reticulum Ca~(2+) -ATPase were significantly reduced in the heart failure-placebo group and which could be significantly attenuated by curcumin (all P＜0.05).Conclusion Curoumin could improve cardiac function via upregulating the expression of sarcoplasmic reticulum Ca~(2+) -ATPas in this model.
