The anti-athetotic effects of heme-L-lysinate in a rabbit model of atherosclerosis.
- Author:
Xiao-bing WANG
1
;
Wan-lin WEI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Atherosclerosis; metabolism; prevention & control; Carbon Monoxide; metabolism; Cholesterol, Dietary; Diet, Atherogenic; Disease Models, Animal; HSC70 Heat-Shock Proteins; genetics; metabolism; Heme; analogs & derivatives; pharmacology; Heme Oxygenase-1; metabolism; Lysine; analogs & derivatives; pharmacology; Male; RNA, Messenger; genetics; Rabbits
- From: Chinese Journal of Cardiology 2010;38(5):450-454
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the anti-atherotic effects of heme-L-lysinate in a rabbit model of atherosclerosis and related machanisms.
METHODSAdult rabbits were treated with 1% cholesterol diet (chol group, n = 8) or 1% cholesterol diet plus heme-L-lysinate (9 mgxkg(-1)xd(-1), Heme group, n = 8) or 1% cholesterol diet plus isotonic Na chloride (Na chloride group, n = 8) for 10 weeks. Eight rabbits fed with normal diet served as normal control. Aortic carbon monoxide (CO) was quantified spectrophotometrically by the formation of carboxyhaemoglobin (HbCO). Aortic heme oxygenase-1 (HO-1) and HSP70 mRNA and protein expressions were detected by RT-PCR and immunohistochemical staining.
RESULTSAortic CO production and HO-1 activity were significantly increased in chol group and Na chloride group compared those in normal control group (P < 0.01). Aortic plaque area was significantly reduced in heme group (26.6% +/- 9.2%) than that in chol group (42.3% +/- 8.7%, P < 0.01). Aortic HO-1 expression, CO production and HSP70 were significantly increased in heme group than those in chol group and Na chloride group (all P < 0.01).
CONCLUSIONSHeme-L-lysinate could attenuate atherosclerotic progression through upregulating HO-1 and HSP70 expression and increasing CO production in this model.
