The anti-atherotic effects of heme-L-lysinate in a rabbit model of atherosclerosis
10.3760/cma.j.issn.0253-3758.2010.05.017
- VernacularTitle:热休克蛋白70在血红素氧合酶/一氧化碳系统抗兔食饵性动脉粥样硬化过程中的效应
- Author:
Xiao-Bing WANG
1
;
Wan-Lin WEI
Author Information
1. 解放军北京军区总医院
- Keywords:
Atherosclerosis;
HSP70 heat-shock proteins;
Heme oxygenase-1;
Carbon monoxide
- From:
Chinese Journal of Cardiology
2010;38(5):450-454
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the anti-atherotic effects of heme-L-lysinate in a rabbit model of atherosclerosis and related machanisms. Methods Adult rabbits were treated with 1 % cholesterol diet (chol group, n =8) or 1% cholesterol diet plus heme-L-lysinate (9 mg · kg-1 · d-1 ,Heme group, n =8) or 1% cholesterol diet plus isotonic Na chloride (Na chloride group, n =8) for 10 weeks. Eight rabbits fed with normal diet served as normal control. Aortic carbon monoxide ( CO ) was quantified spectrophotometrically by the formation of carboxyhaemoglobin ( HbCO). Aortic heme oxygenase-1 ( HO-1) and HSP70 mRNA and protein expressions were detected by RT-PCR and immunohistochemical staining. Results Aortic CO production and HO-1 activity were significantly increased in chol group and Na chloride group compared those in normal control group ( P < 0. 01). Aortic plaque area was significantly reduced in heme group ( 26. 6% ± 9. 2% ) than that in chol group (42. 3% ± 8. 7% , P < 0. 01) . Aortic HO-1 expression, CO production and HSP70 were significantly increased in heme group than those in chol group and Na chloride group (all P < 0. 01 ) . Conclusions Heme-L-lysinate could attenuate atherosclerotic progression through upregulating HO-1 and HSP70 expression and increasing CO production in this model.
