Visfatin upregulates MMP-2 and MMP-9 expressions in human monocytes through activating NF-κB
10.3760/cma.j.issn.0253-3758.2010.05.018
- VernacularTitle:内脂素经核因子κB途径诱导人单核细胞基质金属蛋白酶-2和9表达的实验研究
- Author:
Fen HUANG
1
;
Xiao-Fang XIONG
;
Sha YOU
;
Qiong-Xin WANG
;
He-Song ZENG
Author Information
1. 华中科技大学同济医学院附属同济医院
- Keywords:
Coronary disease;
Matrix metalloproteinase2;
Matrix metalloproteinase9;
NF-kappa B;
Visfatin
- From:
Chinese Journal of Cardiology
2010;38(5):455-459
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of visfatin on the MMP-2 and MMP-9 expressions in human monocytes and related mechanisms. Methods Human monocytes were isolated from blood, the expressions of MMP-2 and MMP-9 at mRNA and protein levels were detected in visfatin stimulated monocytes (0, 100, 200, 400 ng/ml) in the absence and presence of NF-κB inhibitor specific Bay11-7082 by Realtime PCR or Western blot, the MMP-2 and MMP-9 enzyme activity in the culture media was also detected by Gelatin Zymography. The NF-κB protein level and NF-κBp65 expression in visfatin stimulated cells were measured by Western blot and ELISA, respectively. Results Visftain upregulated MMP-2 and MMP-9 expressions in human monocytes in a dose dependent manner. After treatment with visfatin 400 ng/ml for 24 h, comparing with the free visfatin treatment, the protein expressions of MMP-2 and MMP-9 were up-regulated to 1. 644 ± 0.052 and 3. 578 ± 0. 081 (all P < 0. 001); the enzyme activities of MMP-2 and MMP-9 were enhanced by 1. 661 ± 0. 036 ( P < 0. 001) and 1. 662 ± 0. 100 (P < 0. 001 ). NF-κB was also activated in these cells by visaftin and these effects could be significantly attenuated by Bay 11-7082. Visftain induced a dose-dependent ( 100 - 400 ng) increase of NF-kBp65 nuclear translocation from 0. 763 ±0. 056 to 1. 290 ± 0. 065 at 100 and 400 ng/ml, comparing with free visfatin treatment 0. 467 ± 0.046 (all P<0.05). Bay11-7082 decreased the protein expression of MMP-2 and MMP-9 to 1. 183 ± 0. 030 and 2. 024 ± 0. 056 (all P < 0. 001 comparing with 400 ng/ml visfatin treatment). Conclusion Visfatin enhanced the expression and activity of MMP-2 and MMP-9 in human monocytes via activating NF-κB signaling pathway.
