Expression Profile of a Novel Germ Cell-specific Gene, TSCPA, in Mice and Human
10.1007/s11596-009-0502-9
- Author:
YU ZHENDONG
1
;
WU BO
;
TANG AIFA
;
CHEN JING
;
GUO XIN
;
QIN JIE
;
GUI YAOTING
;
CAI ZHIMING
Author Information
1. Department of Clinical Laboratory, Peking University Shenzhen Hospital, Shenzhen 518036, China
- Keywords:
DNA microarray;
TSCPA gene;
spermatogenesis;
Sertoli-cell-only syndrome;
cryp-torchidism
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2009;29(5):535-539
- CountryChina
- Language:Chinese
-
Abstract:
In order to identify novel genes involved in spermatogenesis, testis cDNA samples from Balb/C mice of different postnatal days were hybridized with the whole mouse genome Affymetrix chip to screen the testis-specific genes. The characteristics of the selected genes were analyzed by RT-PCR as well as other bioinformatic tools. A novel differentially expressed testis-specific gene (GenBank Acces-sion No: NM_029042) in the developmental stages of testes was identified, and named TSCPA. Cellular mapping prediction of TSCPA indicated that its protein was probably expressed in nuclei, and one puta-tive domain (aa 332-377) was anchoring domain of cAMP-dependent type Ⅱ PK. The result of subcel-lular localization of GFP-TSCPA fusion protein in Cos-7 cells showed that TSCPA protein was ex-pressed in nuclei. RT-PCR analysis revealed that TSCPA was expressed specifically in mouse and hu-man testis. TSCPA gene was expressed weakly in 21-day-old mouse testis and the expression was in-creased gradually from 38th day to 6th month of mouse testes. No expression of hTSCPA was found in cryptorchidism and Sertoli-cell-only syndrome patients. It was concluded that the expression profile of TSCPA in human and mice indicated that TSCPA might play an important role in spermatogenesis.
