Combined Use of RGD-peptide Modified PLGA and TGF-β1 Gene Transfected MSCs to Improve Cell Biobehaviors in vitro
10.1007/s11596-009-0512-7
- Author:
LI CHANGWEN
1
;
ZHENG QIXIN
;
GUO XIAODONG
;
QUAN DAPING
;
ZHAO JIE
Author Information
1. Department of Orthopedics, Union Hospital Tongii Medical College, Huazhong University of Science and Technology, Wuhan 430022,China
- Keywords:
tissue engineering;
biomaterial;
PLGA;
RGD;
transforming growth factor β;
bone marrow stromal cells
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2009;29(5):592-598
- CountryChina
- Language:Chinese
-
Abstract:
In order to improve the surface properties of PLGA polymer for a better material/cell in-terface to modulate the cells behaviors, we prepared a novel three-block copolymer, PLGA-[ASP-PEG],and immobilized an RGD-containing peptide, Gly-Arg-Gly-Asp-Ser-Pro-Cys (GRGDSPC) on the sur-face of it. Transforming growth factor-131 (TGF-β1) was transfected into bone marrow stromal cells (MSCs) employed as seeded cells. Cell adhesion, spreading, proliferation and differentiation on this material were investigated. The results showed that the cell adhesive ratio on RGD-modified materials was higher than on un-modified materials (P<0.05). The extent of cell spreading was also wider on RGD-modified materials than on un-modified materials. Cell proliferation indices of transfected MSCs were increased as compared with the un-transfected MSCs (P<0.05). The ALP activities in the MSCs cultured with RGD-modified materials were higher than on un-modified materials after 14 days (P<0.05), and those in transfected MSCs were higher than in un-transfected MSCs (P<0.05). It was suggested that the combined use of RGD-modification and TGF-β gene transfection could improve the interaction of biomaterial and cells.
