The Association between RASSF1 Gene Polymorphisms and Lung Cancer Susceptibility among People in Hubei Province of China
10.1007/s11596-009-0522-5
- Author:
XIAO GEQIONG
1
;
ZHANG TAO
;
YAO JIE
;
REN JINGHUA
;
CAO WENMIAO
;
WU GANG
Author Information
1. Cancer Center, Union Hospital Tongji Medical College, Huazhong University of Sciences and Technology, Wuhan 430023, China
- Keywords:
lung cancer;
the RASSF1 gene;
polymorphism,genetic susceptibility,fragment length
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2009;29(5):646-649
- CountryChina
- Language:Chinese
-
Abstract:
The relationship between Ala/Ser polymorphism in 133 codon of exon 3 region of the RASSF 1 gene and genetic susceptibility of lung cancer in Hubei province Han population was inves-tigated by a case-control study. Polymerase chain reaction-restriction fragment length polymorphisrr (PCR-RFLP) technique was adopted to analyze the polymorphism of codon 133 of exon 3 in the RASSF1 gene of 100 pathologically diagnosed lung cancer patients, and 100 healthy controls. The relationship between different genotypes and the susceptibility of lung cancer was analyzed. Among 200 blood samples from Han people in Hubei Province, including 100 from lung cancer patients and 100 from healthy controls, the frequencies of Ala/Ala, Ala/Ser, Ser/Ser genotype of the RASSF1 in lung cancer patients were 83%, 16%, 1%, and those in healthy controls was 93%, 7%, 0% respec-tively, with the difference being statistically significant between two groups (P<0.05). The individu-als with Ala/Ser genotype had higher risk of suffering from lung cancer, with an OR of 2.341, and 95% CI of 1.009-6.393 respectively. It was concluded that RASSFIAla133Ser was a susceptible ge-netic factor of lung cancer. Ala/Ser genotype increased the risk of lung cancer.
