Identification of Trichosporon spp. Strains by Sequencing D1/D2 Region and Sub-typing by Sequencing Ribosomal Intergenic Spacer Region of Ribosomal DNA
10.1007/s11596-009-0524-3
- Author:
ZENG JINGSI
1
;
Cristina Maria de Souza Motta
;
Fukushima KAZUTAKA
;
Takizawa KAYOKO
;
Oliane Maria Correia Magalhes
;
Neves Pereira REJANE
;
Nishimura KAZUKO
Author Information
1. Department of Dermatology and Venereology, Union Hospital, Tongji Medical College, Huazhong University of Science and Tech-nology, Wuhan 430022, China
- Keywords:
Trichonsporon;
large subunit;
D1/D2;
intergenic space 1
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2009;29(5):655-658
- CountryChina
- Language:Chinese
-
Abstract:
To re-identify and further group 25 isolates of Trichosporon spp. identified morphologically previously, sequences of D1/D2 region of large subunit (LSU) of ribosomal DNA (rDNA) of 25 tested strains for identification and those of ribosomal intergenic space 1 (IGS1) region of 11 strains for sub-grouping were detected. The identifications of tested strains were changed except 6 strains. According to the alignment of the IGS1 region, 6 T. asahii isolates tested fell into 4 groups and 5 T. faecale isolates into 3 groups. Polymorphism of 2 T.japonicum isolates was found in 10 positions. With the alignments obtained in this research compared with the relative GenBank entries, it was found that T. asahii, T.faecale and T.japonicum species were divided into 7, 3 and 2 subtypes respectively. Morphological and biophysical methods are not sufficient for Trichosporon spp. identification. Sequencing becomes neces-sary for Trichosporon diagnosis. There is obvious diversity within a species.
