Albumin Modulates the Production of Matrix Metalloproteinases-2 and-9 in Podocytes
10.1007/s11596-009-0607-1
- Author:
FANG ZHAN
1
;
HE FANGFANG
;
CHEN SHAN
;
SUN XIFENG
;
ZHU ZHONGHUA
;
ZHANG CHUN
Author Information
1. Department of Nephrology,1 Union Hospital,Tong]i Medical College,Huazhong University of Science and Technology,Wuhan 430022,China
- Keywords:
podocytes;
albumin;
matrix metalloproteinases;
glomerular basement membrane
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2009;29(6):710-714
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the effects of albumin on the production of matrix metalloproteinases-2 (MMP-2) and matrix metalioproteinases-9 (MMP-9) in podocytes. Podocytes were treated with bovine serum albumin (BSA) at the concentration of 0.1,0.5,1,2 g/L,respectively. Conditioned media were harvested 12,24,48 and 72 h after the treatment. The expression of MMP-2 and MMP-9 was assayed by gelatin zymography,RT-PCR and Western blotting analysis. Our results showed that in comparison with the control group,BSA increased the expression of MMP-2 and MMP-9 mRNA and protein in a dose-and time-dependent manner (P＜0.05). Meanwhile,the enzymatic activities of MMP-2 and MMP-9 in the culture supernatants of podocytes were also increased (P＜0.05). It is concluded that albumin up-regulated the expression of MMP-2 and MMP-9 at gene and protein levels in a time-and dose-dependent manner.
