Skeletal Muscle-derived Stem Cells Exhibit Cardiocyte Competences
10.1007/s11596-009-0614-2
- Author:
LI JIN
1
;
FU DESHENG
;
HONG GUANGXIANG
;
CHEN JIANGHAI
;
KANG HAO
;
CHEN ZHENBIN
Author Information
1. 华中科技大学同济医学院附属协和医院
- Keywords:
skeletal muscle-derived stem cells;
tram-differentiation;
cardiocytes
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2009;29(6):741-744
- CountryChina
- Language:Chinese
-
Abstract:
Adult stem cells from skeletal muscle cells were induced to differentiate into cardiocytes to see if stem cells from another different but histologically-comparable tissues can differentiate to the target cells. Skeletal muscles-derived stem cells (MDSCs) were isolated from adult skeleton muscle tissues by differential adhesion,and immunocytochemically identified by using Sca-1. In order to induce the proliferation but not differentiation of MDSCs,the cells were cultured in Dulbecco's modified Eagle's medium/F12 (DMEM/F12) supplemented with 1:50 B27,20 ng/mL basic fibroblast growth factor (bFGF),20 ng/mL epidermal growth factor (EGF) in a suspension for 6 days. Then these stem cells were treated with 5 μmol/L 5-azacytidine for 24 h in an adherence culture. The characteristics of induced cells were examined by immunocytochemistry,quantitative real time RT-PCR and morphological observation of cell phenotype. Our results showed that the appearance of some cells gradually changed from spindle-shape into polygonal or short-column-shape. Some of these post-treated cells could contract spontaneously and rhythmically. The expression of GATA-4 and cTnT was increased 1 and 2 week(s) after the treatment. And about 16.6% of post-treated cells were cTnT-positive. Therefore,we are led to conclude that skeletal muscle-derived stem cells could differentiate into cardiocyte-like cells,which exhibited some characteristics of cardiocytes.
