Upregulation of Phagocytic Clearance of Apoptotic Cells by Autoimmune Regulator
10.1007/s11596-010-0202-5
- Author:
SHI LIANG
1
;
HU LIHUA
;
LI YIRONG
Author Information
1. Department of Clinical Laboratory, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China
- Keywords:
autoimmune regulator;
apoptosis;
phagocytosis;
racl protein
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2010;30(2):145-148
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the effect of autoimmune regulator(AIRE)on phagocytic clearance of apoptotic cells,a recombinant expression vector containing full-length human AIRE cDNA was transfected into 16HBE cells.After incubation with transfected 16HBE cells,engulfment of apoptotic HL-60 cells induced by camptothecin was detected by myeloperoxidase(MPO)staining.The change in the expression of Rac 1 in transfected 16HBE cells was determined by RT-PCR and Western blotting.The results showed that the phagocytosis percentage of the experimental group,the mock trans-fection group and the negative control group(non-apoptotic cells)was(25.50±3.67)%,(6.25±1.58)%and(1.0±0.67)%,respectively.Moreover,the expressions of Rac 1 mRNA and protein were up-regulated in AIRE-transfected 16HBE cells,suggesting that A1RE may function as a regulator in the phagocytic clearance of apoptotic cells by promoting the expression of Rac 1.
