Change Profiles in Matrix Metalloproteinase-2 and-9 in Induced Endometriosis in Mice

Author: CHEN QIONGHUA ^{1
,

2} ; QIU NAXUAN ; PU DEMIN ; ZHOU YUMIN ; LI TIAN ; YANG HONGYI
Author Information

1. Department of Gynecology and Obstetrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China
2. Department of Gynecology and Obstetrics, the First Affiliated Hospital of Xiamen Medical University, Xiamen 361004, China
Keywords: matrix metalloproteinase; endometriosis; animal model
From: Journal of Huazhong University of Science and Technology (Medical Sciences) 2010;30(2):188-192
CountryChina
Language:Chinese
Abstract: To examine the changes in matrix metalloproteinase-2(MMP-2)and-9(MMP-9)in the development and progression of endometriosis,real time quantitative polymerase chain reaction,enzyme-linked immunoabsorbent assay and gelatin zymography were employed to determine the mRNA and protein levels and activities of MMP-2 and MMP-9 from the first day to the 21st day after the induction in mice with induced endometriosis(experimental group)and sham-operated animals (controls).The results showed that the mRNA and protein levels and activities of the MMP-2 and MMP-9 were significantly increased on the first day after the induction and the level of MMP-2 stayed at a level higher than that in the control group.MMP-9 had two or three peaks during the 21 days,taking place at day 1,4 and 15.It is concluded that the changes in the MMP-2 and MMP-9 might be involved in pathogenesis of endometriosis.