Effects of Progesterone on the Growth Regulation in Classical Progesterone Receptor-negative Malignant Melanoma Cells
10.1007/s11596-010-0220-3
- Author:
FANG XIANFENG
1
,
2
;
ZHANG XUXIN
;
ZHOU MENG
;
LI JIAWEN
Author Information
1. Department of Dermatology, Affiliated Ruikang Hospital Guangxi University of Traditional Chinese Medicine, Nanning 530011, China
2. Department of Dermatology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China
- Keywords:
malignant melanoma;
progesterone;
non-genomic effect
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2010;30(2):231-234
- CountryChina
- Language:Chinese
-
Abstract:
This study investigated the growth-regulating effects of progesterone(Prog)on nPR-negative malignant melanoma cells and the possible mechanisms.A375 and A875 cells were cultured and treated with Prog of different concentrations.For signal transduction pathway studies,the cells were pretreated with Prog receptor antagonist(RU486,1×10 7 mol/L)or MAPK inhibitor (U0126,5×10-6 mol/L)for 1 h and then co-incubated with prog(10-9 mol/L)for another 24 h.Indirect immunofluorescence assay,MTT,flow cytornetry and Western blotting were used for assessing the nPR expression,cell growth,cell apoptosis and ERK1/2 Phosphorylation,respectively.Our results showed that lower progesterone concentration promoted the proliferation of both A375 and A875 cells,but this growth-stimulatory effect decreased at progesterone concentration of 1 × 10-7mol/L or higher.The response could be abolished by MAPK inhibitor U0126,but could not be blocked by progesterone antagonist RU486.Flow cytometry exhibited that high concentration(≥1×10-7 mol/L)progesterone increased the apoptosis of the two cells in a dose-dependent manner.The level of ERK 1/2 phosphorylation was increased by a lower progesterone concentration,but reduced by a higber concentration(1×10-6 mol/L).These results suggest progesterone exerts growth-regulating effects on nPR-negative tumor cells through a non-genomic mechanism.
