Study on the prevalence and genotype of hepatitis E virus among commercial swine population in Eastern and Southern China

VernacularTitle:华东和华南地区商品猪戊型肝炎病毒携带率和基因分型分析
Author: Yu-Gang XIA ¹ ; Yi-Han LU ; An-Qun HU ; Xue QIN ; Xiao-Lian DONG ; Jian-Fu ZHU ; Qing-Wu JIANG ; Ying-Jie ZHENG
Author Information

1. 复旦大学
Keywords: Hepatitis E virus; Prevalence; Genotype; Swine
From: Chinese Journal of Epidemiology 2010;31(7):791-794
CountryChina
Language:Chinese
Abstract: Objective To determine the prevalence and genotype of hepatitis E virus (HEV)among commercial swine population in Eastern and Southern China. Methods Six hundred specimens of swine bile collected from 5 slaughterhouses in Eastern and Southern China from 2007 to 2009 were tested for HEV RNA using nested RT-PCR. PCR products were sequenced for phylogenetic analysis. Results Forty-seven out of the 600 samples (7.83%) were positive for HEV RNA. Based on the 150 nt fragment within HEV ORF2, data from phylogenetic analysis revealed that all the 47 HEV isolates were identified to be genotype Ⅳ, sharing 75.0%-83.4%, 75.0%-84.6%, 71.9%-80.7% and 88.1%-91.5% nucleotide identities with prototype Ⅰ,Ⅱ,Ⅲ and Ⅳ HEV strains respectively while majority of the isolates clustered within their respective isolation sites. Conclusion HEV was widespread in commercial swine population in Eastern and Southern China that raised a serious concern about the safety regarding the consumption of pork products.