Study on the integration site and arrangement of SfII and SfX prophages in Shigella flexneri serotype 2b strains

VernacularTitle:福氏痢疾杆菌2b血清型菌株基因组中SfII和SfX前噬菌体整合位点及排列方式研究
Author: Yi-Ting WANG ¹ ; Qiang-Zheng SUN ; Kai LIU ; Ai-Lan ZHAO ; Yan WANG ; Dong JIN ; Zhen-Jun LI ; Huai-Qi JING ; Chang-Yun YE ; Jian-Guo XU
Author Information

1. 中国疾病预防控制中心传染病预防控制所
Keywords: Shigellaflexneri; Serotype; Prophage; Integration site; Arrangement
From:Chinese Journal of Epidemiology 2010;31(7):800-803
CountryChina
Language:Chinese
Abstract: Objective To study the integration site and arrangement of SfII and SfX prophages in Shigella flexneri serotype 2b strains. Methods A series of primers were designed based on potential integration site of SfII and SfX prophages in Shigella flexneri serotype 2b strains, and PCR were performed for 50 serotype 2b strains to amplify special genes located in host and prophages. PCR products were sequenced to identify integration sites and arrangement of SfII and SfX. Results In all the serotype 2b strains, prophage SfII and SfX were adjacent to each other, and integrated into the thrW tRNA gene of the host, which were located between genes proA and yaiC of host. Prophage SfX was located immediately upstream of prophage SfII in all the detected 50 serotype 2b strains exception for strain 51251. Conclusion This was the first report on the integration site and arrangement of serotype-converting prophages SfII and SfX in Shigella flexneri 2b strains.