Molecular epidemiologic analysis on new emerged type 3 dengue virus in Guangzhou in 2009

VernacularTitle:广州市2009年新出现登革3型病毒的分子流行病学分析
Author: Biao DI ¹ ; Zhi-Jun BAI ; Yu-Lin WANG ; Lei LUO ; Yu CHEN ; Li-Yun JIANG ; Zhi-Cong YANG ; Ming WANG
Author Information

1. 广州市疾病预防控制中心
Keywords: Dengue virus; E gene; Phylogenetic tree
From: Chinese Journal of Epidemiology 2010;31(7):804-807
CountryChina
Language:Chinese
Abstract: Objective To analyze and trace the infection source the envelope(E) gene of the new emerged type 3 dengue virus in Guangzhou in 2009. Methods Sera were collected from patients infected with local dengue fever. Dengue virus was cultured and isolated by C6/36 cells. The whole length E gene was amplified from the positive specimen by RT-PCR, thereby sequenced and phylogenetic tree drawn by neighbor-joining method. Both data on epidemiologic and molecular studies were processed and analysed. Results 7 strains of type 3 dengue virus were isolated from samples of the 19 patients. E gene of these strains was amplified. The complete E genes of 7 strains belonged to 1479 nucleotides in length, encoding a polyprotein of 493 amino acids. Data from the phylogenetic analysis showed that 09/GZ/1081, 09/GZ/1483 and 09/GZ/10806 strains fell within the Southeast Asia/South Pacific group. 09/GZ/10616, 09/GZ/11144, 09/GZ/11194 while 09/GZ/13105 strains fell within the India group. Conclusion The type 3 dengue virus identified in Guangzhou area in 2009 was imported and could be devided into two genotypes.