Preparation of interferon-alpha-loaded poly-L-lactic acid lamellar particles.
- Author:
Xiongwei LI
1
;
Xiaoli CHEN
;
Jialin LIU
;
Weimin CAO
Author Information
1. Chengdu Institute of Organic Chemistry, Chinese Academy of Sciences, Chengdu 610041.
- Publication Type:Journal Article
- MeSH:
Adsorption;
Chemistry, Pharmaceutical;
Drug Carriers;
Interferon-alpha;
chemistry;
pharmacology;
Lactic Acid;
chemistry;
Particle Size;
Polyesters;
Polymers;
chemistry
- From:
Journal of Biomedical Engineering
2002;19(4):559-562
- CountryChina
- Language:Chinese
-
Abstract:
Lamellar particles(lamellae) were prepared by non-solvent precipitation from crystalic poly-L-lactic acid (PLLA). The PLLA lamellae exhibite a diamond or stepped irregular shape with a size range between 3-5 microns. Prepared without any surfactants and dispersing agents, the lamellar particles have clean surface, which is advantageous for the adsorption of proteins. The PLLA lamellar particles adsorbed protein cytokine interferon-alpha (IFN-alpha) with an adsorption efficiency of > or = 95%. The release of loaded IFN could continue for more than 10 days. The cell incubation experiments showed that the PLLA lamellar particles were easy to be phagocytosed by macrophages. The immunological experiments showed that the biological activity of IFN-alpha loaded on the PLLA lamellar particle was effectively retained.
