Clinical significance of changes in quantitative expression of human leukocyte antigen DR in severely burned patients.
- Author:
Ning DONG
1
;
Yong-ming YAO
;
Yu-jue CAO
;
Li-xin HE
;
Jia-ke CHAI
;
Shan XU
;
Zhi-yong SHENG
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Burns; blood; complications; immunology; Female; Flow Cytometry; HLA-DR Antigens; blood; Humans; Lipopolysaccharide Receptors; blood; Male; Middle Aged; Monocytes; metabolism; Multiple Organ Failure; blood; etiology; immunology; Prognosis
- From: Chinese Journal of Surgery 2007;45(11):766-769
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the clinical significance of kinetic changes in quantitative expression of human leukocyte antigen DR (HLA-DR) in severely burned patients.
METHODSThe blood samples of 77 extensively burned patients (>30% of total body surface area) were serially collected in the present study. The expression of HLA-DR on CD14(+) mononuclear cell surface in burned patients were quantified by flow cytometry (using monoclonal antibody, QuantiBRITETM Anti-HLA-DR PE(*)/Anti-Monocyte PerCP-Cy5.5) on days 1, 3, 5, 7, 14, 21 and 28 post burn.
RESULTSThe expressions of HLA-DR on CD14(+) mononuclear cell surface in severely burned patients were significantly lower than those in healthy volunteers from the first day post burn (P < 0.05), and the value of HLA-DR expression was negatively correlated with the burned area (r = -0.7232, P < 0.05). The expression of HLA-DR in patients complicated with multiple organ dysfunction syndrome (MODS) was persistently decreased following major burns, and it was significantly lower than that of non-MODS patients on days 3, 14, 21 and 28 post burn (P < 0.05). The incidence rate of MODS rose markedly along with the lowering of HLA-DR expression, accompanied with poorer prognosis.
CONCLUSIONSExtensive burns could result in marked damage in expression of HLA-DR on CD14(+) mononuclear cell surface and immunologic dysfunction. Quantitative measurement of HLA-DR expression might be of significance in forecasting the development of MODS and prognosis in extensively burned patients.