Melanoma differentiation associated gene-7 and interleukin-24 selectively induces growth arrest and apoptosis in hepatocellular carcinoma cell lines in vitro.
- Author:
Cong-jun WANG
1
;
Zhi-hai PENG
;
Yuan YU
;
Kun CHEN
;
Jian-wei ZHENG
;
Hui-yi HU
;
Wen-wei JI
;
Xin-bo XUE
Author Information
- Publication Type:Journal Article
- MeSH: Adenoviridae; genetics; Apoptosis; genetics; physiology; Carcinoma, Hepatocellular; genetics; pathology; physiopathology; Cell Cycle; genetics; physiology; Cell Line; Cell Line, Tumor; Cell Proliferation; Genetic Vectors; Hepatocytes; cytology; Humans; Interleukins; genetics; physiology; Liver Neoplasms; genetics; pathology; physiopathology; Transfection
- From: Chinese Journal of Surgery 2007;45(17):1202-1205
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of melanoma differentiation associated gene-7/interleukin-24 (mda-7/IL-24) on the hepatocellular carcinoma cell lines and normal liver cell line in vitro.
METHODSHepatocellular carcinoma cell lines HepG2, SMMC7721, Hep3B, MHCC97L, M6 and normal liver cell line L02 were infected with Ad.mda-7. The gene expression of mda-7/IL-24 in these cell lines was confirmed by RT-PCR and ELISA assay. MTT assay and flow cytometry were used to study tumor cell proliferation and cell cycle in vitro. Hoechst staining and cytometry assay after Annexin-V and PI staining were studied to indicate the apoptosis effect.
RESULTSIt was confirmed by RT-PCR that the exogenous mda-7/IL-24 gene expressed in all of these cells. The mda-7/IL-24 protein product was confirmed by assaying the supernatant with ELISA. MTT and apoptosis test indicated mda-7/IL-24 can induce the hepatocellular carcinoma cell lines growth suppression, apoptosis in vitro but not in normal liver cell line L02, cell cycle test revealed mda-7/IL-24 can block cancer cell lines in G2/M but not in L02.
CONCLUSIONSmda-7/IL-24 selectively induces growth suppression, apoptosis in hepatocellular carcinoma lines but not in normal liver cell in vitro.
