Expression of MMP1 mRNA in oral squamous cell carcinoma and paired normal tissues.
- Author:
Xiao-zhi LÜ
1
;
Ya-guang ZOU
;
Xue-min YIN
;
Wan-tao CHEN
;
Chen-ping ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Aged, 80 and over; Carcinoma, Squamous Cell; enzymology; genetics; pathology; Female; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Neoplastic; Humans; Male; Matrix Metalloproteinase 1; genetics; Middle Aged; Mouth Mucosa; enzymology; metabolism; pathology; Mouth Neoplasms; enzymology; genetics; pathology; RNA, Messenger; genetics; metabolism; Reverse Transcriptase Polymerase Chain Reaction
- From: Journal of Southern Medical University 2008;28(8):1362-1364
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the mRNA expression of matrix metalloproteinase 1 (MMP1) gene in oral squamous cell carcinoma (OSCC) and the paired normal tissues.
METHODSThe differential expression of MMP1 mRNA between 30 OSCC and paired normal tissues were detected with reverse transcription-PCR (RT-PCR).
RESULTSThe relative expression level of MMP1 mRNA in the OSCC tissues showed a 3.26-fold increase in comparison with that in the paired normal tissues (4.06-/+0.52 vs 1.24-/+0.17, P<0.0001). In the 30 OSCC tissues, the relative expression level of MMP1 mRNA was higher in histological grade II/III tissues (4.31-/+0.68) than in grade I (3.87-/+0.57) tissues, higher in OSCC in advanced stages (III/IV) than in tumors in early stages (I/II) (4.18-/+0.67 vs 3.65-/+0.53), and also higher in OSCC with cervical lymph node invasion than in those without cervical lymph node invasion (4.32-/+0.71 vs 3.91-/+0.51), but these differences were not statistically significant (P>0.05).
CONCLUSIONMMP1 gene may play a role in local invasion of OSCC, and can serve as a potential biomarker molecule for diagnosis, treatment and prognostic evaluation of OSCC, with also clinical value for OSCC classification.
