High-performance capillary electrophoresis for determining the chemical compositions of Gegen and Fenge extracts.
- Author:
Yong-gang WANG
1
;
Xue-gang SUN
;
Feng-huan WEI
Author Information
- Publication Type:Journal Article
- MeSH: Electrophoresis, Capillary; methods; Isoflavones; analysis; Pueraria; chemistry; Reproducibility of Results
- From: Journal of Southern Medical University 2008;28(8):1407-1408
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a method for analyzing the chemical compositions of Gegen and Fenge extracts using high-performance capillary electrophoresis (HPCE).
METHODSUsing HPCE/DAD, the chemical composition of the extracts was analyzed with the buffer solution of 40 mmol/L borax containing 16.7% methanol, with injection pressure at 137.9 kPa for 5 s, separation voltage at 25 kV in 0-5 min time range and at 22 kV in 5-25 min time range, and the temperature of the capillary of 20 degrees celsius.
RESULTS AND CONCLUSIONThe method for analysis of Gegen and Fenge extracts was established, which identified puerarin and daidzein as the two major components. This simple and rapid analysis method can be used for Gegen and Fenge extract fingerprinting.
