In vitro induced and expanded Epstein Barr virus-specific cytotoxic T lymphocytes can specifically kill nasopharyngeal carcinoma cells.
- Author:
Li-pai CHEN
1
;
Jian-qing HUANG
;
Tong-chong ZHOU
;
Shu-xu ZHANG
;
Jin-long WANG
Author Information
- Publication Type:Journal Article
- MeSH: Antigen-Presenting Cells; cytology; immunology; Antigens, Viral; immunology; B-Lymphocytes; cytology; immunology; virology; Cells, Cultured; Coculture Techniques; Herpesvirus 4, Human; immunology; Humans; Immunotherapy, Adoptive; Nasopharyngeal Neoplasms; immunology; pathology; therapy; T-Lymphocytes, Cytotoxic; cytology; immunology; virology; Tumor Cells, Cultured
- From: Journal of Southern Medical University 2008;28(8):1431-1433
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a method for efficient induction and expansion of Epstein Barr virus (EBV)-specific cytotoxic T lymphocytes (CTL) in vitro and evaluate the possibility of using this strategy for treatment of nasopharyngeal carcinoma (NPC).
METHODSEBV-transformed B lymphoblastoid cells (BLCLs) were used as the antigen stimuli and antigen-presenting cells. EBV-specific CTL was induced by co-culture of the autologous peripheral blood mononuclear cells (PBMCs) and the irradiated BLCLs, and expanded with a cocktail method consisting of OKT-3, irradiated homologous PBMC, and IL-2. The specific activity of the CTL against the NPC cells was measured with MTT assay.
RESULTSEBV-specific CTL was successfully induced and expanded by 600 folds. The killing efficiency of the CTL was 76% for autologous BLCLs, 13% for homologous BLCLs, 51% for autologous NPC cells, and 27% for homologous CNE cell line, and after expansion, the corresponding killing efficiencies were 63%, 25%, 49%, and 33%, respectively. The non-specific killing only slightly increased after the expansion.
CONCLUSIONEBV-specific CTL can be successfully induced and expanded in vitro for specific killing of autologous NPC cells, suggesting the potential of this strategy in the treatment of NPC.
