Establishment of a nude mouse model of nasopharyngeal carcinoma lymph node metastasis and screening of the metastasis-related signature genes.
- Author:
Lei LENG
1
;
Teng-fei LIU
;
Zhong-xi HUANG
;
Wei-bing XIE
;
Kai-tai YAO
Author Information
- Publication Type:Journal Article
- MeSH: Adrenomedullin; genetics; Animals; Caveolin 1; genetics; Cell Line, Tumor; Disease Models, Animal; Gene Expression Profiling; Humans; Interferon Regulatory Factor-1; genetics; Lymphatic Metastasis; Mice; Mice, Inbred BALB C; Mice, Nude; Nasopharyngeal Neoplasms; genetics; pathology; Neoplasm Transplantation; Reverse Transcriptase Polymerase Chain Reaction; Transplantation, Heterologous
- From: Journal of Southern Medical University 2008;28(9):1519-1522
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a nude mouse model of nasopharyngeal carcinoma (NPC) lymph node metastasis and screen the signature genes associated with the metastasis.
METHODSThe NPC 5-8F-EGFP cells were inoculated into nude mice, from which a 5-8F-LN cell line with lymph node metastasis potential was obtained. The lymphatic metastasis-related signature genes of breast cancer and head and neck squamous cell carcinoma were screened by data mining method.
RESULTSThe NPC cell lines 5-8F and 6-10B showed 307 differentially expressed genes by microarray analysis, from which 20 overlapping genes were identified, and 3 overexpressed genes were found with probable metastasis potential, namely the ADM, IRF1, and CAV1 genes. Quantitative RT-PCR validated the data mining results in the 5-8F-EGFP, 6-10B-EGFP, NP69, and 5-8F-LN cell lines. The 3 NPC cell lines 5-8F-EGFP, 6-10B-EGFP and 5-8F-LN showed significantly higher expressions of IRF1 than NP69 cells (P=0.008, 0.022, and 0.006, respectively. The expression level of CAV1 in 5-8F-EGFP cells was significantly higher than that in 6-10B-EGFP cells (P=0.014), but ADM expression showed no significant difference between the 4 cell lines.
CONCLUSIONSIRF1 may play an important role in the progression of NPC. The overexpression of CAV1 in 5-8F-EGFP cells can be associated with the high metastatic potential of the cells.
