Establishment of a tight tetracycline-controlled HCV-C double transgenic mouse model.
- Author:
Li-fang SHUAI
1
;
Bo-heng TANG
;
Ruo-shuang ZHANG
;
Yong ZHAO
;
Guo-zhu YANG
;
Xi-gu CHENG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apolipoproteins E; genetics; Blotting, Southern; Breeding; Crosses, Genetic; Female; Gene Expression Regulation, Viral; drug effects; Hepacivirus; genetics; immunology; Hepatitis C Antigens; genetics; immunology; Male; Mice; Mice, Transgenic; Polymerase Chain Reaction; Tetracycline; pharmacology; Trans-Activators; genetics; Viral Core Proteins; genetics
- From: Journal of Southern Medical University 2008;28(9):1530-1533
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a tight tetracycline-controlled HCV-C double transgenic mouse model.
METHODSBy crossbreeding of ApoE-rtTA-tTS transgenic mice with TRE-HCV-C transgenic mice, the double transgenic mice were produced in the F1 generation. The presence of HCV-C and tTS gene in the F1 generation was confirmed by PCR, followed by further identification and quantification of the transgene using Southern blot hybridization. The expression of HCV-C in the liver of the mouse model was detected immunohistochemically.
RESULTS AND CONCLUSIONTwo transgenic mice were obtained, which contained ApoE-rtTA-tTS and TRE-HCV-C genes in the genome. Five founders contained HCV-C gene as confirmed by PCR and Southern blot hybridization. The tight tetracycline-controlled system may facilitate further study of HCV-C gene expression and gene therapy of hepatic cellular carcinoma.