Preliminary study of the association between human thrombospondin and gastric cancer.
- Author:
Yi-hui LI
1
;
Wei-zhi YANG
;
Jin-zhong CHEN
;
Zhi-qian HU
Author Information
- Publication Type:Journal Article
- MeSH: Biomarkers, Tumor; genetics; metabolism; Cell Line, Tumor; Gene Expression Profiling; methods; Gene Expression Regulation, Neoplastic; Humans; Protein Binding; Reverse Transcriptase Polymerase Chain Reaction; Stomach Neoplasms; genetics; metabolism; pathology; Thrombospondins; genetics; metabolism
- From: Journal of Southern Medical University 2008;28(9):1546-1549
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the possible role of human thrombospondin (hPWTSR) in gastric cancer and explore its potential to serve as the target for gastric cancer diagnosis and intervention.
METHODSUsing pLexA-hPWTSR as the bait, a premade pB42AD-based fetal brain cDNA library was constructed to identify the interacting proteins. The expression pattern of hPWTSR in gastric cancer tissues and a gastric cancer cell line was observed to investigate the correlation between hPWTSR expression and the biological behaviors of the tumor. The possibility of hPWTSR as a potential gastric cancer marker was evaluated.
RESULTSFifty-seven independent clones were isolated from 107 clones screened. Sequence analysis indicated that the 57 positive clones represented the products of 12 genes. A RT-PCR-based expression pattern revealed that the expression of hPWTSR in gastric cancer tissues and a gastric cancer cell line was lower than that in the corresponding normal tissues, but no mutations were identified by the subsequent sequence analysis.
CONCLUSIONShPWTSR interacts with adhesion-related proteins and tumor-related genes, and its expression is lowered in gastric cancer tissues and gastric cancer cell line. hPWTSR might play a role in gastric cancer development, especially in metastasis and might be used as a potential gastric cancer marker. The exact functions of hPWTSR and its potential clinical value still await further study.