Establishment of a mouse model of biliary obstruction and its dynamic observations.
- Author:
Shou-hua ZHANG
1
;
Cai-xian LIAO
;
Chun-xing ZHANG
;
Hong DENG
;
Hang ZHU
;
Lei LEI
;
Chen YAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cholestasis, Extrahepatic; pathology; Common Bile Duct; pathology; surgery; Disease Models, Animal; Female; Ligation; Liver; pathology; Mice; Mice, Inbred BALB C
- From: Journal of Southern Medical University 2008;28(9):1579-1581
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a mouse model of biliary obstruction.
METHODSSixty-four Balb/c mice were divided into experimental group and control group. Obstructive jaundice was induced in the mice in the experimental group by common bile duct ligation. The level of the common bile duct diameter, WBC, LYM MID, LYM%, MID% and ALT, AST, TBIL, DBIL, IBIL, ALP and CHOL were measured 12 h and 1, 2 ,3, 4, 5, and 7 days after the ligation. The morphological changes in the liver were also observed.
RESULTSThe level of common bile duct diameter, WBC, LYM, MID, LYM%, MID% and ALT, AST, TBIL, DBIL, ALP and CHOL all underwent changes with time following certain patterns.
CONCLUSIONThe jaundice manifestation of this model is similar to that of patients with biliary obstruction, and this model may provide a reliable model for studying the mechanism of obstructive jaundice.
