Effect of small interfering RNA-mediated silencing of CD158b expression on the cytotoxicity of NK cells against the allogeneic dendritic cells.
- Author:
Dong-lin CAO
1
;
Wei CHEN
;
Wei-li MA
Author Information
- Publication Type:Journal Article
- MeSH: Bone Marrow Transplantation; immunology; methods; Cytotoxicity, Immunologic; immunology; Dendritic Cells; cytology; immunology; Flow Cytometry; Gene Silencing; Graft vs Host Disease; immunology; Humans; Killer Cells, Natural; cytology; immunology; RNA Interference; RNA, Messenger; biosynthesis; genetics; RNA, Small Interfering; genetics; Receptors, KIR; metabolism; Receptors, KIR2DL3; genetics; metabolism; Reverse Transcriptase Polymerase Chain Reaction
- From: Journal of Southern Medical University 2008;28(9):1603-1613
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effects of small interfering RNA (siRNA)-mediated silencing of CD158b expression on the efficiency of the natural killer (NK) cells in killing allogeneic dendritic cells.
METHODSAfter the knockdown of CD158b by CD158b -SiRNA, the CD158b mRNA expression in natural killer cells was examined by qRT-PCR and the CD158b protein expression by flow cytometer. The cytotoxic activity of RNAi-NK cells and normal NK cells against the allogeneic dendritic cells was detected by LDH release assay.
RESULTSThe CD158b mRNA expression and its protein expression were decreased significantly in the NK cells by CD158b siRNA (P/0.05). The cytotoxic activities of alloreactive NK cells generated by RNAi CD158b expression against allogeneic dendritic cells were increased significantly.
CONCLUSIONSilencing CD158b gene can inhibit the NK cell CD158B mRNA and protein expression. Alloreactive NK cells generated by RNAi CD158b expression have the potential for use in interventions of GVHD.
