Analysis of circulating DNA level in the plasma of cervical cancer patients.
- Author:
Ting GUAN
1
;
Xin-yu GUO
;
Chang-lan YE
;
Yue-hua JIANG
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Carcinoma, Squamous Cell; blood; diagnosis; Cervical Intraepithelial Neoplasia; blood; diagnosis; DNA, Neoplasm; blood; Female; Humans; Middle Aged; Sensitivity and Specificity; Uterine Cervical Neoplasms; blood; diagnosis
- From: Journal of Southern Medical University 2008;28(9):1663-1667
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine the plasma DNA level and investigate its clinicopathological significance in women with cervical cancers.
METHODSBlood samples were collected from 42 cervical cancer patients, 20 patients with cervical intraepithelial neoplasia grade III (CINIII) and 20 healthy women. The plasma DNA was extracted using a commercial kit and detected by a fluorescentmeter.
RESULTSThe mean plasma DNA level in stage I cervical cancer patients was 12.78-/+5.58 ng/ml, significantly higher than that in CINIII patients (8.10-/+3.06 ng/ml) and normal controls (7.60-/+3.87 ng/ml) (P=0.001). The mean DNA level in stage II-III patients was 17.99-/+7.81 ng/ml, significantly higher than that in stage I patients (P=0.02). No significant difference was found in plasma DNA level between CINIII patients and the normal controls (P>0.05). When the cut-off for diagnosis of cervical cancer was 15.70 ng/ml, the sensitivity, specificity, positive predictive value and negative predictive value were 38.10%, 92.50%, 84.21% and 58.73%, respectively.
CONCLUSIONPlasma DNA level is closely related with malignant transformation and development of cervical cancer, and may become a useful means for differential diagnosis of cervical cancer.
