Effect of the sera of rabbits fed with Tongxinluo on MMP-9 and TIMP-1 expression and secretion in U937 monocyte-derived macrophages.
- Author:
Jin-lai LIU
1
;
Qiang-feng ZHU
;
Bao-shun HAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Atherosclerosis; blood; etiology; Cholesterol, Dietary; administration & dosage; Drugs, Chinese Herbal; pharmacology; Enzyme-Linked Immunosorbent Assay; Humans; Macrophages; drug effects; metabolism; Male; Matrix Metalloproteinase 9; biosynthesis; genetics; RNA, Messenger; biosynthesis; genetics; Rabbits; Reverse Transcriptase Polymerase Chain Reaction; Serum; Tissue Inhibitor of Metalloproteinase-1; biosynthesis; genetics; U937 Cells
- From: Journal of Southern Medical University 2008;28(9):1703-1706
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of the sera of rabbits fed with Tongxinluo on the expression and secretion of matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in U937 monocyte-derived macrophages.
METHODSAtherosclerosis was induced in rabbits by high-cholesterol feeding, and the serum was obtained from the rabbits after administration of the aqueous solution of Tongxinluo or simvastatin by gavage. U937 monocyte-derived macrophages were incubated with the sera at different concentrations for 24 hours, and the changes in MMP-9 and TIMP-1 gene expression and secretion were detected by RT-PCR and enzyme-linked immunosorbent assay, respectively.
RESULTSThe serum of rabbits fed with Tongxinluo concentration-dependently inhibited the expression and secretion of MMP-9 in U937 macrophages, but did not affect TIMP-1 expression or secretion.
CONCLUSIONTongxinluo may stabilize the atherosclerotic plaques by inhibiting the expression and secretion of MMP-9.
