A simple method for assessment of RNA integrity in laser capture microdissection samples.
- Author:
Ying-fang TIAN
1
;
Zhao-ming WEI
;
Xin-lin CHEN
;
Fen QIU
;
Xin-li XIAO
;
Qian-yan KANG
;
Bo-feng ZHU
;
Yu-mei TIAN
;
Jun-feng ZHANG
;
Yong LIU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Capillaries; pathology; Cerebral Cortex; blood supply; pathology; Lasers; Male; Microdissection; methods; Neurons; pathology; RNA; analysis; isolation & purification; Rats; Rats, Sprague-Dawley
- From: Journal of Southern Medical University 2008;28(10):1782-1785
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a simple method for assessment of RNA integrity in laser capture microdissection (LCM) samples.
METHODSThe total RNA were isolated from the LCM samples and the sections before and after microdissection and examined by agarose gel electrophoresis. Real-time PCR was employed to assess the RNA from LCM samples, and the quantity of RNA was theoretically estimated according to the average total RNA product in mammalian cells (10 ng/1000 cells).
RESULTSWhen the total RNA from the sections before and after microdissection was intact, the RNA from LCM samples also had good quality, and the 28S and 18S rRNAs were visualized by ethidium bromide staining. Real-time PCR also showed good RNA quality in the LCM samples.
CONCLUSIONA simple method for quantitative and qualitative assessment of the RNA from LCM samples is established, which can also be applied to assessment of DNA or proteins in LCM samples.
