Inhibitory effects of saikosaponin a on rat hippocampal astrocyte activation in vitro.
- Author:
Wei XIE
1
;
Jia LIN
;
Zuo-wen ZHANG
;
Ye ZHOU
;
Yong BAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Animals, Newborn; Astrocytes; cytology; Cell Proliferation; drug effects; Cells, Cultured; Female; Glial Fibrillary Acidic Protein; biosynthesis; Glutamic Acid; pharmacology; Hippocampus; cytology; Male; Oleanolic Acid; analogs & derivatives; pharmacology; Rats; Rats, Sprague-Dawley; Saponins; pharmacology
- From: Journal of Southern Medical University 2008;28(10):1798-1801
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of saikosaponin a (SSa) on Glu-activated hippocampal astrocytes of rats.
METHODSNeonatal rat (1-3 days) hippocampal astrocytes were obtained and divided into control group, L-Glu activation group and SSa groups with SSa treatment at 5, 2.5, and 1.25 mg/L. The cell proliferation, cell cycle changes, and expression of glial fibrillary acidic protein (GFAP) after the treatments were assessed with MTT assay, flow cytometry and Western blotting, respectively.
RESULTSIn comparison with Glu-activation group, SSa treatment resulted in significant inhibition of the cell proliferation, cell division and GFAP expression in the Glu-activated astrocytes (P < 0.05). SSa at 2.5 mg/L showed the strongest inhibitory effects against astrocyte activation and maintained nearly normal level of astrocyte activation in comparison with the control group (P > 0.05).
CONCLUSIONSGlu-induced activation of rat hippocampal astrocytes can be inhibited by SSa, whose antiepileptic effects is probably mediated by inhibition of hippocampal astrocyte activation.
