Application of surface enhanced laser desorption/ionization time-of-flight mass spectrometry in the diagnosis of leukemia.
10.7534/j.issn.1009-2137.2014.05.016
- Author:
Hua HUANG
1
;
Lin-Xing CHEN
2
;
Mei-Shan LIN
1
;
Jing-Yu HUANG
1
Author Information
1. Department of Clinical Laboratory, The Second Hospital Affiliated to Shantou University Medical College,Shantou 515041,Guangdong Province, China.
2. Department of Clinical Laboratory, The Second Hospital Affiliated to Shantou University Medical College,Shantou 515041,Guangdong Province, China. E-mail:5280599@sina.com.
- Publication Type:Journal Article
- MeSH:
Biomarkers, Tumor;
analysis;
Humans;
Leukemia;
diagnosis;
Molecular Weight;
Peptide Mapping;
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization;
methods
- From:
Journal of Experimental Hematology
2014;22(5):1273-1277
- CountryChina
- Language:Chinese
-
Abstract:
This study was purposed to find new biomarkers and to establish protein finger print model for diagnosis of leukemia. A total of 40 leukemia samples and 37 healthy control samptes were tested by surface enhance laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF- MS). The data of spectra were analyzed by bioinformatics tools like Biomarker Patterns 5.0 and discriminant analysis to establish diagnostic mode1. The results showed that 22 protein features were stably detected by protein fingerprint, The detective model combined with 3 biomarkers (m/z 4650, 8609 and 11660) could differentiate leukemia with sensitivity of 97.5% (39/40) and specificity of 91.9%(34/37). It is concluded that the detective model established by 3 protein features may be a novel method for diagnosis of leukemia.
