Effects of bortezomib combined with 5-azacytidine on the apoptosis of K562 cells and expression of SHIP mRNA.
10.7534/j.issn.1009-2137.2014.05.020
- Author:
Zhi-Qiang JIA
1
;
Yu-Tao WEI
2
;
Yu-Lian WEI
3
;
Wei SU
2
;
Chun-Xia YU
2
;
Jin TAO
4
;
Hong-Qi RONG
2
Author Information
1. Department of Hemotology,Baoding Municipal First Hospital, Baoding 071000, Hebei Province, China. E-mail: jzq980315@163.com.
2. Department of Hemotology,Baoding Municipal First Hospital, Baoding 071000, Hebei Province, China.
3. Sixth Department of Internal Medicine, Hebei Sixth People's Hospital, Baoding 071000, Hebei Province, China.
4. Department of General Surgery, The Children's Hospital of Baoding City, Baoding 071000, Hebei Province, China.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
drug effects;
Azacitidine;
pharmacology;
Boronic Acids;
pharmacology;
Bortezomib;
Cell Proliferation;
Humans;
Inositol Polyphosphate 5-Phosphatases;
K562 Cells;
Phosphoric Monoester Hydrolases;
genetics;
Pyrazines;
pharmacology;
RNA, Messenger;
biosynthesis
- From:
Journal of Experimental Hematology
2014;22(5):1291-1294
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to investigate the effects of bortezomib combined with 5-azacytidine on the apoptosis of K562 cells and expressiom of SHIP mRNA. The K562 cells were cultured and treated with different concentrations of bortezomib, 5-azacytidine or their combination for 24 hours. Then, the expression of SHIP mRNA was detected by RT-PCR,the cell proliferation was analyzed by using MTT assay and flow cytometry. The results showed that 5-20 nmol/L bortezomib could effectively inhibit the proliferation of K562 and this inhibitory effect gradually enhanced along with the increase of bortezomib concentration, the group of bortezomib combined with 5-azacytidine showed more inhibitory effect on K562 cells than that of bortezomib or 5-azacytidine alone.The bortezomib could promote the apoptosis of K562 cells in a dose-dependent manner,and this apoptotic effect was higher in group of bortezomib combined with 5-azacytidine than that in group of bortezomib or 5-azacytidine alone.Bortezomib could down-regulated the expression of SHIP mRNA in a dose-dependent manner,and this down-requlated effect was higher in group of bortezomib combined with 5-azacytidine than that in group of bortezomib or 5-azacytidine alone.It is concluded that bortezomib and 5-azacytidine can induce apoptosis by inhibiting the expression of SHIP mRNA in K562 cells.The combination of bortezomib with 5-azacytidine displays a synergetic effect.
