Effect of AZD8330 on proliferation and apoptosis of multiple myeloma cells.
10.7534/j.issn.1009-2137.2014.05.024
- Author:
Yao YAO
1
;
Yue-Ping BIAN
1
;
Dan-Dan XIA
1
;
Bin PAN
1
;
Ming-Shan NIU
1
;
Kai ZHAO
1
;
Ling-Yu ZENG
1
;
Kai-Lin XU
2
Author Information
1. Department of Hematology, the Affiliated Hospital of Xuzhou Medical College, Xuzhou 221006, Jiangsu Province, China.
2. Department of Hematology, the Affiliated Hospital of Xuzhou Medical College, Xuzhou 221006, Jiangsu Province, China. E-mail: kailinxu_xzmc@126.com.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
drug effects;
Caspase 3;
Cell Cycle;
Cell Line, Tumor;
Cell Proliferation;
drug effects;
Cyclin E;
Dihydropyridines;
pharmacology;
Humans;
Multiple Myeloma;
pathology;
Oncogene Proteins;
Protein Kinase Inhibitors;
pharmacology
- From:
Journal of Experimental Hematology
2014;22(5):1311-1315
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to investigate the effect of MEK inhibitor AZD8330 on proliferation and apoptosis of multiple myeloma IM9 and NCI-H929 cell lines and its possible mechanism. These two cell line cells were exposed to different concentrations of AZD8330 for 48 h. The CCK-8 assay was used to detect cell viability and the IC50 value at 48 h. These above-mentioned IM9 and NCI-H929 cells were treated with 5,10 and 100 nmol/L of AZD8330, then the change of cell cycle was analysed by flow cytometry with PI staining. The Wester blot was used to detect the expression levels of cyclin D and cyclin E, and multiple myeloma cells were treated with 10, 100, 1000 and 2000 nmol/L of AZD8330, the AnnexinV/7-AAD double staining was used to analyse cell apoptosis and the Western blot was used to detect the expression level of caspase-3. The results showed that AZD8330 could significantly inhibit the cell viability of IM9 and NCI-H929 cell lines in a time-and dose-dependent manner, the IC50 value (48 h) of IM9 and NCI-H929 were 19.88 ± 2.7 nmol/L and 29.3 ± 2.03 nmol/L respectively, these two cell lines were arrested on G1 phase of cell cycle, the apoptosis cells increased along with enhancement of AZD8330 concentration, and the expression level of cleaved caspase-3 protein was up-regulated. It is concluded that AZD8330 can efficiently inhibit the proliferation of NCI-H929 and IM9 cell lines, and induce apoptosis, suggesting that the AZD8330 may be a potential chemotherapeutic candidate for multiple myeloma therapy.
