Influence of donor mouse age on the establishment of murine acute graft versus host disease model after allogenic hematopoietic stem cell transplantation.
10.7534/j.issn.1009-2137.2014.05.035
- Author:
Bo TANG
1
,
2
;
Heng ZHU
3
;
Xi-Mei LI
1
;
Ya-Nan CHU
4
;
Yuan-Lin LIU
3
;
Fan ZHOU
5
;
Yi ZHANG
6
Author Information
1. Training Base of Postgraduates, Liaoning Medical College and General Hospital of Shenyang Military Command, Shenyang 110016, Liaoning Province, China
2. Department of Cell Biology, Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850, China.
3. Department of Cell Biology, Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850, China.
4. Department of Cell Biology, Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850, China
5. Training Base of Postgraduates, Liaoning Medical College and General Hospital of Shenyang Military Command, Shenyang 110016, Liaoning Province, China. E-mail: zhoufan611@sina.com.cn.
6. Department of Cell Biology, Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850, China. E-mail: zhangyi612@hotmail.com.
- Publication Type:Journal Article
- MeSH:
Acute Disease;
Aging;
Animals;
Bone Marrow;
Bone Marrow Cells;
Bone Marrow Transplantation;
Disease Models, Animal;
Graft vs Host Disease;
Hematopoietic Stem Cell Transplantation;
Male;
Mice;
Mice, Inbred BALB C;
Spleen;
Transplantation, Homologous
- From:
Journal of Experimental Hematology
2014;22(5):1365-1370
- CountryChina
- Language:Chinese
-
Abstract:
This study was purposed to elucidate the influence of donor mouse age on the establishment of murine acute graft versus host disease (aGVHD) model after allogenic hematopoietic stem cell transplantation. The male mice with 2-week-old, 10-week-old and 18-week-old mice (BALB/cH-2Kb) were taken as donors. The 8-week-old mice (BALB/c, H-2Kd) were selected as recipients. Each group animals were irradiated with 7.5 Gy (60)Co for total body, the recipient mice were injected intravenously with 1 × 10⁷ bone marrow cells and 1 × 10⁷ spleenoctyes from various donors in 4-5 hours after irradiation. Mouse transplant characteristics and survival were observed every day. The white blood cell number in peripheral blood of each group were counted at day 5, 10, 15, 20, 25 and 30 after transplantation. Furthermore, the pathological damage in the liver, spleen, lung and intestines were evaluated by sectioning and in situ hematoxylin-eosin (HE) staining. The results showed that compared with the 2-week-old and 10-week-old donor groups, mice received bone marrow (BM) cells and splenocytes from 18-week-old mice showed higher incidence of aGVHD, lower clinical GVHD scores and suffered from diarrhea, ruffled hair, a hunched posture, and diminished body weight. In contrast, mice received BM cells and splenocytes from 2-week-old donor mice indicated attenuated GVHD symptoms and survived longer. The histo-pathological analysis in 18-week-old donor group demonstrated the most serious pathological damage in the liver, spleen, lung and intestines. It is concluded that the donor age has been confired to have an obvious influence on the establishment of murine aGVHD model. This study lay an important foundation for establishing animal models and may be helpful for further study.
