Detecting the abnormal expression of PDGFRA gene in eosinophilia by FISH.
10.7534/j.issn.1009-2137.2014.05.037
- Author:
Yan-Fang WANG
1
;
Lian-Yong XI
1
;
Hua WANG
1
;
Fei DONG
1
;
Wei ZHAO
1
;
Xiao-Yan KE
2
Author Information
1. Department of Hematology, Peking University Third Hospital, Beijing 100191, China.
2. Department of Hematology, Peking University Third Hospital, Beijing 100191, China. E-mail: xiaoyank@yahoo.com.
- Publication Type:Journal Article
- MeSH:
Chromosomes, Human, Pair 4;
Eosinophilia;
metabolism;
Humans;
In Situ Hybridization, Fluorescence;
Oncogene Proteins, Fusion;
Receptor, Platelet-Derived Growth Factor alpha;
genetics;
Translocation, Genetic
- From:
Journal of Experimental Hematology
2014;22(5):1377-1380
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to investigate the abnormal expression of PDGFRA gene in eosinophilia by FISH. Translocations of PDGFRA gene in 13 patients with eosinophilia were detected by using 4q12 three-color probe and FISH technology. Fifteen people were used as control to establish the normal cut-off value of fluorescence signal of PDGFRA. The results indicated that 1 out of 13 patients with eosinophilia was corrected and was diagnosed as CML. The fusion gene of FIP1L1-PDGFRA (F/P) was found in 2 patients and the positive rate of F/P fusion gene detected by probe 4q12 was 17% in the 12 patients with eosinophilia. Other translocation forms involving PDGFRA gene were not found. It is concluded that a variety of translocation forms of PDGFRA gene can be detected in patients with eosinophilia by using 4q12 three-color probe and FISH technology, which can provide important information for assessing diagnosis and treatment.
