Commonly used cre transgenic mice and their applications in hematopoietic system.
10.7534/j.issn.1009-2137.2014.05.049
- Author:
Lu-Yun PENG
1
;
Tao CHENG
2
;
Wei-Ping YUAN
1
Author Information
1. State Key Laboratory of Experimental Hematology, Institute of Hematology & Blood Disease Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China.
2. State Key Laboratory of Experimental Hematology, Institute of Hematology & Blood Disease Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China. E-mail: wpyuan@ihcams.ac.cn.
- Publication Type:Journal Article
- MeSH:
Animals;
Hematopoietic Stem Cells;
cytology;
metabolism;
Integrases;
Mice;
Mice, Transgenic
- From:
Journal of Experimental Hematology
2014;22(5):1442-1447
- CountryChina
- Language:Chinese
-
Abstract:
Cre-lox recombination system consists of two elements: Cre recombinase enzyme and lox sites. Cre recombinase can recombine the lox site sequences by specifically detecting and cutting them. The direction and position of lox sites determine the functional effects of Cre enzyme such as deletion, inversion or chromosomal translocation. The hematopoietic system of mouse consists of multi-lineages and various developmental stage hematopoietic cells that are differentiated from hematopoietic stem cells (hematopoietic stem cells, HSC). The hematopoietic stem cells are maintained in the bone marrow microenvironment (niche). Currently, a variety of floxed conditional-knockout mice, recognized by Cre-lox recombination system, are used for the study of the hematopoietic system. This review summarizes the commonly used Cre transgenic mice and their applications in the study of hematopoietic system.
