Research advances on DNA extraction methods from peripheral blood mononuclear cells.
10.7534/j.issn.1009-2137.2014.05.060
- Author:
Xiao-Ying WANG
1
;
Chen-Xi YU
2
Author Information
1. Department of Nutrition & Food Hygiene, School of Public Health, Southeast University, Nanjing 210009, Jiangsu Province, China. E-mail: wxy@seu.edu.cn.
2. Department of Clinical Medicine, School of Medicine, Southeast University, Nanjing 210009, Jiangsu Province, China.
- Publication Type:Journal Article
- MeSH:
DNA;
isolation & purification;
Genomics;
Humans;
Immunomagnetic Separation;
Leukocytes, Mononuclear;
chemistry;
Molecular Biology;
methods;
Phenol
- From:
Journal of Experimental Hematology
2014;22(5):1495-1498
- CountryChina
- Language:Chinese
-
Abstract:
DNA extraction is a basic technology of molecular biology. The purity and the integrality of DNA structure are necessary for different experiments of gene engineering. As commonly used materials in the clinical detection, the fast, efficient isolation and extraction of genomic DNA from peripheral blood mononuclear cells is very important for the inspection and analysis of clinical blood. At present, there are many methods for extracting DNA, such as phenol-chloroform method, salting out method, centrifugal adsorption column chromatography method (artificial methods), magnetic beads (semi-automatic method) and DNA extraction kit. In this article, a brief review of the principle for existing DNA blood extraction method, the specific steps and the assessment of the specific methods briefly are summarized.
